Coupled BIA-MS at the origin of two recent articles…

For several years now, the Technology Cluster has been developing an original strategy for the identification of interaction partners.  This approach associates BIA (Biomolecular Interaction Analysis) technology to measure molecular interactions and SPR (Surface Plasmon Resonance) with Mass Spectrometry.  The principle consists in immobilising the molecule of interest (DNA, RNA, protein, etc.) on a special surface (Sensorchip), injecting real-time a complex mixture, measuring the specificity of the interaction and recovering the partner(s) that have been fixed. As a second stage, the proteins thus recovered are identified using mass spectrometry.

 

This coupling of BIA and MS was exploited to identify the partners of P36, a peptide fragment derived from ALK (Cf. Aubry et al., Cell Death and Disease, on the Bibliography page), and to identify ITAFs, proteins that regulate the activity of IRES in FGF-1 (Cf. Ainaoui et al., Plos One on the Bibliography page).